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  5. The Ultrastructural Analysis of Human Colorectal Cancer Stem Cell-Derived Spheroids and Their Mouse Xenograft Shows That the Same Cells Types Have Different Ratios

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Article
en
2021

The Ultrastructural Analysis of Human Colorectal Cancer Stem Cell-Derived Spheroids and Their Mouse Xenograft Shows That the Same Cells Types Have Different Ratios

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en
2021
Vol 10 (9)
Vol. 10
DOI: 10.3390/biology10090929

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Michela Relucenti
Michela Relucenti

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Michela Relucenti
Federica Francescangeli
Maria Laura De Angelis
+8 more

Abstract

Spheroids from primary colorectal cancer cells and their mice xenografts have emerged as useful preclinical models for cancer research as they replicate tumor features more faithfully as compared to cell lines. While 3D models provide a reliable system for drug discovery and testing, their structural complexity represents a challenge and their structure-function relationships are only partly understood. Here, we present a comparative ultrastructural and flow citometric analysis of patient colorectal cancer-derived spheroids and their mice xenografts. Ultrastructural observations highlighted that multicellular spheroids and their xenografts contain the same cancer cell types but with different ratios, specifically multicellular spheroids were enriched in cells with a stem-like phenotype, while xenografts had an increased amount of lipid droplets-containing cells. The flow cytometric analysis for stem cell marker and activity showed enrichment of stem-like cells presence and activity in spheroids while xenografts had the inverse response. Our results evidence the effects on cancer cells of different in vitro and in vivo microenvironments. Those differences have to be paid into account in designing innovative experimental models for personalized drug testing.

How to cite this publication

Michela Relucenti, Federica Francescangeli, Maria Laura De Angelis, Vito D’Andrea, Selenia Miglietta, Emanuela Pilozzi, Xiaobo Li, Alessandra Boe, Rui Chen, Ann Zeuner, Giuseppe Familiari (2021). The Ultrastructural Analysis of Human Colorectal Cancer Stem Cell-Derived Spheroids and Their Mouse Xenograft Shows That the Same Cells Types Have Different Ratios. , 10(9), DOI: https://doi.org/10.3390/biology10090929.

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Publication Details

Type

Article

Year

2021

Authors

11

Datasets

0

Total Files

0

Language

en

DOI

https://doi.org/10.3390/biology10090929

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