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Get Free AccessChromatographic parameter assessments for RP-HPLC-UV method development for the simultaneous analysis of artemether and azithromycin for the pharmaceutical analysis of a rectal coformulation currently under development for the treatment of malaria infected children. Using methanol based mobile phase for the analysis of both artemether and azithromycin provided a more robust method in terms of resolution and peak symmetry. The method validated for suppository used 80% methanol and 20% phosphate buffer 15 mM at pH 9. The UV detection was at 210 nm. The accuracy profiles indicated a method validation between 80–120% for both active pharmaceutical ingredients. The preparation process of the suppository was validated based on theoretical values of artemether and azithromycin present in the formulation; active pharmaceutical ingredients were homogenously distributed within the suppository.
Karen Gaudin, Tina Kauss, Alexandra Gaubert, Vincent Viaud, Jean‐Pierre Dubost, Piero Olliaro, Sir Nicholas White, Pascal Millet (2011). Simultaneous Determination of Artemether and Azithromycin in Suppositories by Reversed Phase HPLC. Analytical Letters, 44(16), pp. 2732-2743, DOI: 10.1080/00032719.2011.553014.
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Type
Article
Year
2011
Authors
8
Datasets
0
Total Files
0
Language
English
Journal
Analytical Letters
DOI
10.1080/00032719.2011.553014
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