Global characterization of bioactive compounds of <em>Vicia faba </em>L. by-product by means of UHPLC-MS<sup>2</sup><strong><sup> </sup></strong>

Currently, by-products produced from the agro-industries practices could be a key source of functional and bioactive components; which could be used due to their nutritional and added- value properties. New aspects concerning the use of these wastes as by-products in production of food additives or supplements have recently gained increasing interest. In this sense, the present study describes an in-depth characterization of phytochemical compounds from hydro-methanolic extract of broad beans pods by using high-performance liquid chromatography linked with quadrupole-time-of-flight tandem mass spectrometry. The utilized analytical technique provided the tentative identification more than 140 phenolic and other phytochemical compounds in the extract, most of which have not been described up to now in broad beans pods. Thus, more than 90 phytochemicals (phenolic acids, flavonoids, iridoids, lignans, and terpenoids derivatives) are reported herein in broad beans pods for the first time. The data obtained demonstrate that vegetal by-product from the food industry could potentially be utilized as a promising source of bio-active ingredients to design new nutraceuticals and functional foods with a valuable future market. Moreover, the got data may form a basis for future bioavailability and quantitative studies, which will be a next step in our research work.