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Get Free AccessThe CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.
Ping Lv, Fei Su, Fangyuan Chen, Chunxue Yan, Dandan Xia, Hongyu Sun, Shanshan Li, Zhiqiang Duan, Changle Ma, Hui Zhang, Mugui Wang, Xiaomu Niu, Jian Kang Zhu, Jinshan Zhang (2023). Genome editing in rice using <scp>CRISPR</scp>/Cas12i3. , 22(2), DOI: https://doi.org/10.1111/pbi.14192.
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Type
Article
Year
2023
Authors
14
Datasets
0
Total Files
0
Language
en
DOI
https://doi.org/10.1111/pbi.14192
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