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Get Free AccessAbstract A contribution of DNA methylation to defense against invading nucleic acids and maintenance of genome integrity is uncontested; however, our understanding of the extent of involvement of this epigenetic mark in genome-wide gene regulation and plant developmental control is incomplete. Here, we knock out all five known DNA methyltransferases in Arabidopsis , generating DNA methylation-free plants. This quintuple mutant exhibits a suite of developmental defects, unequivocally demonstrating that DNA methylation is essential for multiple aspects of plant development. We show that CG methylation and non-CG methylation are required for a plethora of biological processes, including pavement cell shape, endoreduplication, cell death, flowering, trichome morphology, vasculature and meristem development, and root cell fate determination. Moreover, we find that DNA methylation has a strong dose-dependent effect on gene expression and repression of transposable elements. Taken together, our results demonstrate that DNA methylation is dispensable for Arabidopsis survival but essential for the proper regulation of multiple biological processes.
Huan Huang, Mariem Bradai, Cheng Zhao, You Yin, Jun Ma, Lun Zhao, Rosa Lozano‐Durán, Jian Kang Zhu, Li He (2022). DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development. , 13(1), DOI: https://doi.org/10.1038/s41467-022-28940-2.
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Type
Article
Year
2022
Authors
9
Datasets
0
Total Files
0
Language
en
DOI
https://doi.org/10.1038/s41467-022-28940-2
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