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Get Free AccessKeyhole limpet hemocyanin (KLH) is a glycosylated multi-subunit metalloprotein that elicits a strong nonspecific immune activation, thus inducing both cellular and humoral immune responses. The exceptional immunogenicity of this protein can be leveraged to vaccinate mice against self-antigens that otherwise would not induce an autoimmune response. This protocol describes the covalent conjugation of KLH with acyl-coenzyme A-binding protein (ACBP), the autovaccination of mice with ACBP-KLH conjugate together with a potent adjuvant, and the detection of the produced anti-ACBP autoantibodies. For complete details on the use and execution of this profile, please refer to Bravo-San Pedro et al. (2019c).
Léa Montégut, Hui Chen, José Manuel Bravo‐San Pedro, Omar Motiño, Isabelle Martins, Guido Guido Kroemer (2022). Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin. , 3(1), DOI: https://doi.org/10.1016/j.xpro.2021.101095.
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Type
Article
Year
2022
Authors
6
Datasets
0
Total Files
0
Language
en
DOI
https://doi.org/10.1016/j.xpro.2021.101095
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