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  5. Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin

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Article
en
2022

Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin

0 Datasets

0 Files

en
2022
Vol 3 (1)
Vol. 3
DOI: 10.1016/j.xpro.2021.101095

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Guido Guido Kroemer
Guido Guido Kroemer

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Léa Montégut
Hui Chen
José Manuel Bravo‐San Pedro
+3 more

Abstract

Keyhole limpet hemocyanin (KLH) is a glycosylated multi-subunit metalloprotein that elicits a strong nonspecific immune activation, thus inducing both cellular and humoral immune responses. The exceptional immunogenicity of this protein can be leveraged to vaccinate mice against self-antigens that otherwise would not induce an autoimmune response. This protocol describes the covalent conjugation of KLH with acyl-coenzyme A-binding protein (ACBP), the autovaccination of mice with ACBP-KLH conjugate together with a potent adjuvant, and the detection of the produced anti-ACBP autoantibodies. For complete details on the use and execution of this profile, please refer to Bravo-San Pedro et al. (2019c).

How to cite this publication

Léa Montégut, Hui Chen, José Manuel Bravo‐San Pedro, Omar Motiño, Isabelle Martins, Guido Guido Kroemer (2022). Immunization of mice with the self-peptide ACBP coupled to keyhole limpet hemocyanin. , 3(1), DOI: https://doi.org/10.1016/j.xpro.2021.101095.

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Publication Details

Type

Article

Year

2022

Authors

6

Datasets

0

Total Files

0

Language

en

DOI

https://doi.org/10.1016/j.xpro.2021.101095

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