0 Datasets
0 Files
Get instant academic access to this publication’s datasets.
Yes. After verification, you can browse and download datasets at no cost. Some premium assets may require author approval.
Files are stored on encrypted storage. Access is restricted to verified users and all downloads are logged.
Yes, message the author after sign-up to request supplementary files or replication code.
Join 50,000+ researchers worldwide. Get instant access to peer-reviewed datasets, advanced analytics, and global collaboration tools.
✓ Immediate verification • ✓ Free institutional access • ✓ Global collaborationJoin our academic network to download verified datasets and collaborate with researchers worldwide.
Get Free AccessAnthocyanidin synthase (ANS; EC 1.14.11.19) is one of the key enzymes in the biosynthesis of anthocyanins and proanthocyanidins, which contribute to color and mouthfeel of grape berry and wine. Previous studies concerning the ANS gene in reproductive organs like grape berries mainly focused on gene expression and regulation at transcriptional level. Little was reported about translational controlling and cellular localization. In the present study, the full ANS cDNA with 1068bp was cloned from grape berries (Vitis vinifera L. Cabernet Sauvignon) via PCR, the recombinant protein was obtained by prokaryotic expression and a polyclonal antibody against grape berry ANS was prepared. On this basis, the tissue and subcellular localization of ANS during grape berry development were investigated by using the techniques of immunohistochemical, immuno-electron microscopy and confocal microscopy. The results showed that ANS distributed in both exocarp and mesocarp cells of grape berries and the changes of ANS signals correspond well to the temporal accumulation patterns of ANS gene transcript and ANS protein during grape berry development. Furthermore a trio cytoplasmic/plastid/nuclear subcellular localization of ANS was observed. This work will provide new insight for the regulation of different branch pathways leading to diverse flavonoid compounds in grape berry.
Huiling Wang, Wei Wang, Ping Zhang, Qiu‐Hong Pan, Jicheng Zhan, Weidong Huang (2010). Gene transcript accumulation, tissue and subcellular localization of anthocyanidin synthase (ANS) in developing grape berries. Plant Science, 179(1-2), pp. 103-113, DOI: 10.1016/j.plantsci.2010.04.002.
Datasets shared by verified academics with rich metadata and previews.
Authors choose access levels; downloads are logged for transparency.
Students and faculty get instant access after verification.
Type
Article
Year
2010
Authors
6
Datasets
0
Total Files
0
Language
English
Journal
Plant Science
DOI
10.1016/j.plantsci.2010.04.002
Access datasets from 50,000+ researchers worldwide with institutional verification.
Get Free Access
